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The GST-PRMT1 fusion protein or GST protein was mixed with His-UBE2m fusion protein overnight under gentle rotation at 4 C and then incubated with GST beads (78602, Thermo Fisher Scientific) at 4 C for 3 h

Although gastrointestinal tolerability was not explicitly evaluated, metformin is associated with such adverse effects in clinical settings, a factor relevant for translational consideration